PtBio and Hiroshima University Granted Patent for Off-Target Risk Analysis Method and System: 2026-07-02

PtBio and Hiroshima University have jointly been granted a patent for an off-target risk analysis method, off-target risk analysis system, program, and recording medium for genome editing. PtBio is a participating company in the Center of Innovation

for Bio-Digital Transformation(Bio-DX).

This patented technology powers DOLPHiN, PtBio’s proprietary off-target risk analysis simulation system. DOLPHiN is an advanced analytical platform designed to ensure the safety of genome editing tools.

The patent is one of the research outcomes of the Center of Innovationfor Bio-DX under the R&D project titled “Development of a Digitally Driven End-to-End Genome Editing Platform for Industrial Applications.”

Conventional off-target prediction methods generally rely on comparing the target organism's entire genome sequence (reference genome) to identify potential off-target sites. In contrast, this patented technology can predict the likelihood of potential off-target risks using only the information of the intended genome editing target sequence itself.

This enables high-risk target sequences to be effectively filtered during the initial screening stage. Using PtBio’s proprietary algorithm, the system quantitatively evaluates and identifies target sequences with a low likelihood of causing off-target effects, regardless of the organism's genome.

The technology is expected to be particularly valuable in situations such as:

• Genome editing of organisms whose complete reference genomes are unavailable, or when a rapid assessment of the safety of designed target sequences is required.

• Designing safer target sequences when actual genomic structures differ substantially from available reference genomes due to individual genetic variation or other factors. 

Through this technology, the Center of Innovationfor Bio-DX aims to reduce analytical costs while maximizing safety in industrial genome editing. By enabling safer and more efficient genome editing across a wide range of organisms, the Hub seeks to contribute to the advancement of a sustainable bioeconomy.

■Patent Information

• Patent Number: Patent No. 7878661  

• Title of Invention: Off-Target Risk Analysis Method, Off-Target Risk Analysis System, Program, and Recording Medium   

• Date of Registration: June 15, 2026

• Patent Specification: Available here.